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Addgene

pYH71
(Plasmid #217381)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217381 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mcdB
  • Species
    Synthetic
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYH71 was a gift from Anthony Vecchiarelli (Addgene plasmid # 217381 ; http://n2t.net/addgene:217381 ; RRID:Addgene_217381)

  • For your References section:

    An experimental framework to assess biomolecular condensates in bacteria. Hoang Y, Azaldegui CA, Ghalmi M, Biteen JS, Vecchiarelli AG. bioRxiv [Preprint]. 2023 Mar 23:2023.03.22.533878. doi: 10.1101/2023.03.22.533878. 10.1101/2023.03.22.533878 PubMed 36993636
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