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Addgene

pBS-AMVpl
(Plasmid #21738)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21738 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2964
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AMV-pA50
  • Insert Size (bp)
    96

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer M13for20
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-AMVpl was a gift from Henry Lester (Addgene plasmid # 21738 ; http://n2t.net/addgene:21738 ; RRID:Addgene_21738)

  • For your References section:

    Channel gating governed symmetrically by conserved leucine residues in the M2 domain of nicotinic receptors. Labarca C, Nowak MW, Zhang H, Tang L, Deshpande P, Lester HA. Nature. 1995 Aug 10. 376(6540):514-6. 10.1038/376514a0 PubMed 7637783
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