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Addgene

GW1CMV-Perceval Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: SphI/BglII double digest and SphI/EcoRI double digest.

We expected the following band sizes for this digest: ~5.7 + 1.9kb bands for the SphI/BglII digest and ~4.8+2.7kb bands for the SphI/EcoRI digest. We see the expected pattern.