GW1CMV-Perceval
(Plasmid #21737)

• Purpose: can be used to monitor the ATP:ADP ratio during live-cell imaging
• Depositing Lab: Gary Yellen
• Publication: Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

Backbone

• Vector backbone: pGW1-CMV
• Backbone manufacturer: British Biotech
• Backbone size w/o insert (bp): 5076
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Perceval (fluorescent ATP:ADP sensor)
• Species: Synthetic
• Insert Size (bp): 1934
• Mutation: improved Kozak sequence
• Tag / Fusion Protein:
  • 5'UTR (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-N (rev) gives 5' third
• 3′ sequencing primer: EGFP-C (fwd) gives middle third

Resource Information

• Supplemental Documents:
  • Perceval
• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry