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Addgene

pAcBac1-BacMam-4x-PyOtR
(Plasmid #217364)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217364 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcBac1
  • Backbone manufacturer
    derived from pFastBac-Dual from Invitrogen.
  • Backbone size w/o insert (bp) 8024
  • Total vector size (bp) 9411
  • Vector type
    Mammalian Expression, Insect Expression ; baculoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Gentamicin, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4 copies of pyrrolysyl-tRNA mutant PyOtR
  • Alt name
    4xU6-PytOR
  • Alt name
    4xPytOR
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1387
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (not destroyed)
  • 3′ cloning site AvrII (destroyed during cloning)
  • 5′ sequencing primer CCAATAGGCCGAAATCGGCAAAATCC
  • 3′ sequencing primer GCAGCTTATAATGGTTACAAATAAAGCAATAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcBac1-BacMam-4x-PyOtR was a gift from Abhishek Chatterjee (Addgene plasmid # 217364 ; http://n2t.net/addgene:217364 ; RRID:Addgene_217364)

  • For your References section:

    Enhanced Directed Evolution in Mammalian Cells Yields a Hyperefficient Pyrrolysyl tRNA for Noncanonical Amino Acid Mutagenesis. Jewel D, Kelemen RE, Huang RL, Zhu Z, Sundaresh B, Malley K, Pham Q, Loynd C, Huang Z, van Opijnen T, Chatterjee A. Angew Chem Int Ed Engl. 2024 Jan 26:e202316428. doi: 10.1002/anie.202316428. 10.1002/anie.202316428 PubMed 38279536
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