MAP4K3-GFP K45E
(Plasmid #21734)

• Depositing Lab: L. Miguel Martins
• Publication: Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ():.

Backbone

• Vector backbone: pcDNA3.1/CT-GFP-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6157
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MAP4K3
• Alt name: GLK
• Alt name: MAPKKKK3
• Alt name: RAB8IPL1
• Species: H. sapiens (human)
• Insert Size (bp): 2619
• Mutation: Mutation of the ATP-binding site (K45E). This version of the kinase is catalytically inactive
• GenBank ID: BC071579
• Entrez Gene: MAP4K3 (a.k.a. GLK, MAPKKKK3, MEKKK 3, MEKKK3, RAB8IPL1)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ cloning site: T/A cloning (unknown if destroyed)
• 3′ cloning site: T/A cloning (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: GFP Reverse (5 ́-GGGTAAGCTTTCCGTATGTAGC-3 ́)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry