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Addgene

pNS49 (enAsCas12a-KRAB piggyBac)
(Plasmid #217335)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217335 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene plasmid # 133568
  • Backbone manufacturer
    Mario Capecchi
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    enAsCas12a
  • Species
    Acidaminococcus
  • Mutation
    E174R/S542R/K548R
  • Promoter CAG
  • Tags / Fusion Proteins
    • 6xMycNLS (N terminal on insert)
    • HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.18.558350 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNS49 (enAsCas12a-KRAB piggyBac) was a gift from Luke Gilbert (Addgene plasmid # 217335 ; http://n2t.net/addgene:217335 ; RRID:Addgene_217335)

  • For your References section:

    Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations. Hsiung CC, Wilson CM, Sambold NA, Dai R, Chen Q, Teyssier N, Misiukiewicz S, Arab A, O'Loughlin T, Cofsky JC, Shi J, Gilbert LA. Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0. 10.1038/s41587-024-02224-0 PubMed 38760567
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