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Addgene

pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG
(Plasmid #217321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac HTB
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLRP1
  • Alt name
    Lewis rat NLRP1 (allele 5)
  • Species
    R. norvegicus (rat)
  • Mutation
    S969A
  • Promoter polyhedrin
  • Tags / Fusion Proteins
    • His-TEV (N terminal on backbone)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer polyhedrin-fwd
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG was a gift from Yifan Wang (Addgene plasmid # 217321 ; http://n2t.net/addgene:217321 ; RRID:Addgene_217321)
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