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Addgene

pMRX-IBU-pSu9-HaloTag7-SNAP
(Plasmid #217319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-IBU
  • Backbone size w/o insert (bp) 7652
  • Total vector size (bp) 5945
  • Vector type
    Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
  • Species
    Neurospora crassa
  • Insert Size (bp)
    1707
  • GenBank ID
  • Promoter CMV
  • Tags / Fusion Proteins
    • HaloTag (C terminal on insert)
    • SNAP (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IBU-pSu9-HaloTag7-SNAP was a gift from Noboru Mizushima (Addgene plasmid # 217319 ; http://n2t.net/addgene:217319 ; RRID:Addgene_217319)

  • For your References section:

    Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. Yang Z, Yoshii SR, Sakai Y, Zhang J, Chino H, Knorr RL, Mizushima N. EMBO J. 2024 Nov;43(22):5613-5634. doi: 10.1038/s44318-024-00272-5. Epub 2024 Oct 17. 10.1038/s44318-024-00272-5 PubMed 39420095
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