pLSPR32
(Plasmid #217317)

• Purpose: GAL7(p)-mNeonGreen:amdS fusion reporter
• Depositing Lab: James Fraser
• Publication: Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567. doi: 10.3390/jof10080567.

Backbone

• Vector backbone: pSDMA57
• Backbone manufacturer: Stratagene
• Total vector size (bp): 10011
• Vector type: Yeast Expression
• Selectable markers: amdS

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Acetamidase
• Alt name: amdS
• Species: Aspergillus nidulans
• Insert Size (bp): 4330
• Promoter: GAL7
• Tag / Fusion Protein:
  • mNeonGreen (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pLSPR32 - GAL7-mNeonGreen-amdS fusion reporter.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains the mNeonGreen amdS2 fusion reporter in the multicloning site of pSDMA57 which is a modified pBluescript II -SK backbone vector. The vector is designed integration into the Cryptococcus neoformans genome via homologous recombination with the Safe Haven site. This construct contains the native C. neoformans GAL7 promoter upstream of the fusion reporter.