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Addgene

pLSPR25
(Plasmid #217310)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217310 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSDMA57
  • Backbone manufacturer
    Stratagene
  • Total vector size (bp) 9109
  • Vector type
    Yeast Expression
  • Selectable markers
    amdS

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Acetamidase
  • Alt name
    amdS
  • Species
    Aspergillus nidulans
  • Insert Size (bp)
    3105
  • Tag / Fusion Protein
    • mNeonGreen (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains the mNeonGreen amdS2 fusion reporter in the multicloning site of pSDMA57 which is a modified pBluescript II -SK backbone vector. The vector is designed integration into the Cryptococcus neoformans genome via homologous recombination with the Safe Haven site. This construct lacks a promoter, instead has a unique 8 bp SwaI endonuclease restriction site to allow for easy 'drop-in' of desired sequence upstream of the fusion reporter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLSPR25 was a gift from James Fraser (Addgene plasmid # 217310 ; http://n2t.net/addgene:217310 ; RRID:Addgene_217310)
  • For your References section:

    A Chimeric ORF Fusion Phenotypic Reporter for Cryptococcus neoformans. Phillips-Rose LS, Yu CK, West NP, Fraser JA. J Fungi (Basel). 2024 Aug 12;10(8):567. doi: 10.3390/jof10080567. 10.3390/jof10080567 PubMed 39194893