pLSPR25
(Plasmid #217310)

• Purpose: Promoterless (SwaI Cutsite) mNeonGreen:amdS fusion reporter
• Depositing Lab: James Fraser
• Publication: Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567. doi: 10.3390/jof10080567.

Backbone

• Vector backbone: pSDMA57
• Backbone manufacturer: Stratagene
• Total vector size (bp): 9109
• Vector type: Yeast Expression
• Selectable markers: amdS

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Acetamidase
• Alt name: amdS
• Species: Aspergillus nidulans
• Insert Size (bp): 3105
• Tag / Fusion Protein:
  • mNeonGreen (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pLSPR25 - SwaI-mNeonGreen-amdS fusion reporter.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains the mNeonGreen amdS2 fusion reporter in the multicloning site of pSDMA57 which is a modified pBluescript II -SK backbone vector. The vector is designed integration into the Cryptococcus neoformans genome via homologous recombination with the Safe Haven site. This construct lacks a promoter, instead has a unique 8 bp SwaI endonuclease restriction site to allow for easy 'drop-in' of desired sequence upstream of the fusion reporter.