pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)
(Plasmid
#217305)
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PurposesgRNA BFP + Puromycin
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217305 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
- Total vector size (bp) 8904
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Vector typeCRISPR, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepU6-sgRNA, Ef1alpha-Puro-T2A-BFP
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gRNA/shRNA sequenceGACCAGGATGGGCACCACCC
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SpeciesSynthetic
- Promoter U6, Ef1alpha
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer actgacgggcacatgca (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP) was a gift from Luke Gilbert (Addgene plasmid # 217305 ; http://n2t.net/addgene:217305 ; RRID:Addgene_217305)
