pLM433
(Plasmid
#217293)
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Purpose(Empty Backbone) For creating CyanoTag vectors by Golden Gate cloning (BspQI version)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLM434
- Backbone size (bp) 8400
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Vector typeSynthetic Biology
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Tag
/ Fusion Protein
- mNeonGreen - 3x FLAG (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLM433 was a gift from Luke Mackinder (Addgene plasmid # 217293 ; http://n2t.net/addgene:217293 ; RRID:Addgene_217293)
Map uploaded by the depositor.
