9.5-Luc
(Plasmid
#21728)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21728 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBlueScript II KS(+)
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 5600
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep21 promoter deletion mutant
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Alt namep21, WAF1
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SpeciesH. sapiens (human)
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Insert Size (bp)137
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Entrez GeneCDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
9.5-Luc was a gift from Wafik El-Deiry (Addgene plasmid # 21728 ; http://n2t.net/addgene:21728 ; RRID:Addgene_21728) -
For your References section:
AP2 inhibits cancer cell growth and activates p21WAF1/CIP1 expression. Zeng YX, Somasundaram K, el-Deiry WS. Nat Genet. 1997 Jan . 15(1):78-82. 10.1038/ng0197-78 PubMed 8988173