4-Luc
(Plasmid #21725)

• Depositing Lab: Wafik El-Deiry
• Publication: Zeng et al Nat Genet. 1997 Jan . 15(1):78-82.

Backbone

• Vector backbone: pBlueScript II KS(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p21 promoter deletion mutant
• Alt name: p21, WAF1
• Species: H. sapiens (human)
• Insert Size (bp): 899
• Entrez Gene: CDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry