pROSA26-5'
(Plasmid #21715)

• Depositing Lab: Philippe Soriano
• Publication: Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Backbone

• Vector backbone: pBluescript KS (-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3027
• Vector type: for use with mouse targeting vector pROSA26-1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ROSA26-5' insert
• Alt name: ROSA26
• Species: M. musculus (mouse)
• Insert Size (bp): 132
• Entrez Gene: Gt(ROSA)26Sor (a.k.a. Gtrgeo26, Gtrosa26, R26, ROSA26, Thumpd3as1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Probe for detecting targeted integrations by Southern blots. It is a small probe so users should wash at low stringency, eg. 1xSSC at 65 degrees.

We recommend you order this plasmid along with pROSA26-1 and SAbgeo as a "ROSA26 targeting kit".

For more information visit -
http://research.mssm.edu/soriano/lab