pROSA26-1
(Plasmid #21714)

• Depositing Lab: Philippe Soriano
• Publication: Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Backbone

• Vector backbone: pBluescript KS (-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4486
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ROSA26
• Alt name: gene trap ROSA26
• Species: M. musculus (mouse)
• Insert Size (bp): 5360
• Entrez Gene: Gt(ROSA)26Sor (a.k.a. Gtrgeo26, Gtrosa26, R26, ROSA26, Thumpd3as1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Addgene Notes:
  • User digest of plasmid 21714 pROSA26-1
• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

General ROSA26-1 targeting vector. For more information visit - http://research.mssm.edu/soriano/lab

We recommend you order this plasmid along with SAbgeo and pROSA26-5' as a "ROSA26 targeting kit".

Please note that although the full plasmid sequence indicates PmeI does not cut the plasmid, there is a PmeI site somewhere in the plasmid. See Notes from Addgene link above for more information.