SA-betageo
(Plasmid #21709)

• Depositing Lab: Philippe Soriano
• Publication: Friedrich et al Genes Dev. 1991 Sep . 5(9):1513-23.

Backbone

• Vector backbone: pBluescript KS (-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Mouse gene trapping
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SAbgeo
• Alt name: bifunctional lacZ/neomycin phosphotransferase gene
• Species: bgeo fusion protein

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSAbgeo, gene trap vector, use this as a source of SA for targeting ROSA26.

For more information visit -
http://research.mssm.edu/soriano/lab

We recommend you order this plasmid along with pROSA26-1, pROSA26-5' as a "ROSA26 targeting kit".