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pAAV-U6-sasgRNACMV- mCherry-WPREpA
(Plasmid #217015)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217015 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAVCMV- sadCas9-VP64-pA
  • Total vector size (bp) 4963
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • gRNA/shRNA sequence
    None
  • Species
    Discosoma sea anemones
  • Promoter CMV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pAAV-U6-sasgRNACMV-mCherry-WPREpA was cloned by replacing the CMV-sadCas9-VP64-pA cassette in pAAVCMV-sadCas9-VP64-pA backbone with that of U6-sasgRNA-CMV-mCHerry-WPREpA cassette from pLTR-1120-MP177-U6-sasgRNA-mcherry (a kind gift from B. Huang at UCSF).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S. aureus sgRNAs can be cloned using the In-Fusion HD-cloning kit (Clontech) into the Bst XI and Xho I
restriction enzyme sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-U6-sasgRNACMV- mCherry-WPREpA was a gift from Nadav Ahituv (Addgene plasmid # 217015 ; http://n2t.net/addgene:217015 ; RRID:Addgene_217015)

  • For your References section:

    CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency. Matharu N, Rattanasopha S, Tamura S, Maliskova L, Wang Y, Bernard A, Hardin A, Eckalbar WL, Vaisse C, Ahituv N. Science. 2018 Dec 13. pii: science.aau0629. doi: 10.1126/science.aau0629. 10.1126/science.aau0629 PubMed 30545847
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