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pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
(Plasmid #217014)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217014 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-30a (+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1623
  • Mutation
    CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
  • Entrez Gene
    CDK7 (a.k.a. CAK, CAK1, CDKN7, HCAK, MO15, STK1, p39MO15)
  • Entrez Gene
    CDK2 (a.k.a. CDKN2, p33(CDK2))
  • Tag / Fusion Protein
    • C3 protease cleavable GST tag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) was a gift from Manjeet Mukherjee (Addgene plasmid # 217014 ; http://n2t.net/addgene:217014 ; RRID:Addgene_217014)

  • For your References section:

    Protein engineering enables a soakable crystal form of human CDK7 primed for high-throughput crystallography and structure-based drug design. Mukherjee M, Day PJ, Laverty D, Bueren-Calabuig JA, Woodhead AJ, Griffiths-Jones C, Hiscock S, East C, Boyd S, O'Reilly M. Structure. 2024 Jun 9:S0969-2126(24)00188-6. doi: 10.1016/j.str.2024.05.011. 10.1016/j.str.2024.05.011 PubMed 38870939
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