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pFastBac dual_Human full length CDK7(W132R, S164D, T170E)
(Plasmid #217013)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217013 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac dual
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Gentamicin, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDK7(W132R, S164D, T170E)
  • Alt name
    cdk7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1056
  • Mutation
    CDK7(W132R, S164D, T170E)
  • Entrez Gene
    CDK7 (a.k.a. CAK, CAK1, CDKN7, HCAK, MO15, STK1, p39MO15)
  • Tag / Fusion Protein
    • TEV cleavable his tag

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac dual_Human full length CDK7(W132R, S164D, T170E) was a gift from Manjeet Mukherjee (Addgene plasmid # 217013 ; http://n2t.net/addgene:217013 ; RRID:Addgene_217013)

  • For your References section:

    Protein engineering enables a soakable crystal form of human CDK7 primed for high-throughput crystallography and structure-based drug design. Mukherjee M, Day PJ, Laverty D, Bueren-Calabuig JA, Woodhead AJ, Griffiths-Jones C, Hiscock S, East C, Boyd S, O'Reilly M. Structure. 2024 Jun 9:S0969-2126(24)00188-6. doi: 10.1016/j.str.2024.05.011. 10.1016/j.str.2024.05.011 PubMed 38870939
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