pET30-GBF-E6N
(Plasmid #21699)

• Depositing Lab: James Baleja
• Publication: Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.

Backbone

• Vector backbone: pET30
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Protein G domain B1 fused N-terminal zinc binding domain of human papillomavirus type 16 (residues 2-74)
• Alt name: E6
• Alt name: protein G
• Species: Human papillomavirus type 16
• Insert Size (bp): 609
• Mutation: GB1 (1-56aa) fusion tag with mutations at three positions D22N/D36R/E42K was fused to N-terminal zinc binding domain of HPV-16 E6 (residues 2-74) with a linker AAPRGS (including a thrombin cleavage site AAPR).
• Entrez Gene: E6 (a.k.a. HpV16gp1)
• Tag / Fusion Protein:
  • Protein G B1 domain (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pET-30a(+) Restriction Sites

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The whole E6 gene was cloned into the vector, however a stop codon was introduced at amino acid 75, creating the truncated form.