Skip to main content
Addgene

pET30-GBF-E6
(Plasmid #21696)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21696 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET30
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142
  • Alt name
    E6
  • Alt name
    protein G
  • Species
    Human papillomavirus type 16
  • Insert Size (bp)
    609
  • Mutation
    GB1 (1-56aa) fusion tag with mutations at three positions: D22N/D36R/E42K. HPV-16 E6 with mutations at four positions (C80S/C97S/C111S/C140S) and deletion of amino acids 143-151. The linker between GB1 and E6 is AAPRGS (including a thrombin cleavage site AAPR).
  • Entrez Gene
    E6 (a.k.a. HpV16gp1)
  • Tag / Fusion Protein
    • Protein G B1 domain (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-GBF-E6 was a gift from James Baleja (Addgene plasmid # 21696 ; http://n2t.net/addgene:21696 ; RRID:Addgene_21696)

  • For your References section:

    Determinants of stability for the E6 protein of papillomavirus type 16. Liu Y, Cherry JJ, Dineen JV, Androphy EJ, Baleja JD. J Mol Biol. 2009 Mar 6. 386(4):1123-37. 10.1016/j.jmb.2009.01.018 PubMed 19244625
Related items: