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Addgene

pST39-Mega'-deltaBRS
(Plasmid #216947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216947 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST39
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Mega' with knockout of GvpB, GvpR and GvpS
  • Alt name
    GvpB is also known as GvpA2
  • Species
    Synthetic; Priestia megaterium

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.07.23.550228v1 for bioRxiv preprint.

Please note: This plasmid contains an A269P mutation in GvpL and a 41 basepair insertion in the backbone. These mutations are not expected to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST39-Mega'-deltaBRS was a gift from George Lu (Addgene plasmid # 216947 ; http://n2t.net/addgene:216947 ; RRID:Addgene_216947)
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