pET30-hDlg PDZ2
(Plasmid #21692)

• Depositing Lab: James Baleja
• Publication: Liu et al Biochemistry. 2007 Sep 25. 46(38):10864-74.

Backbone

• Vector backbone: pET30
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hDlg/SAP97 PDZ2 domain
• Species: H. sapiens (human)
• Insert Size (bp): 291
• Mutation: human hDlg/SAP97 PDZ2 domain (residues 318-405)
• Entrez Gene: DLG1 (a.k.a. DLGH1, SAP-97, SAP97, hdlg)
• Tag / Fusion Protein:
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pET-30a(+) Restriction Sites

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a second His tag that is C-terminal on the vector after the first His tag.It is however not in frame with the insert and other His tag.