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Addgene

pDONR223-VPS35
(Plasmid #21689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2391
  • Vector type
    Gateway entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Invitrogen stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vacuolar Protein Sorting 35 homolog
  • Alt name
    VPS35
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2391
  • Mutation
    last nucleotide of stop codon removed for c-terminal tagging; no stop codon.
  • GenBank ID
    NM_018206
  • Entrez Gene
    VPS35 (a.k.a. MEM3, PARK17)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site att (not destroyed)
  • 3′ cloning site att (not destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223-VPS35 was a gift from Lynda Chin (Addgene plasmid # 21689 ; http://n2t.net/addgene:21689 ; RRID:Addgene_21689)

  • For your References section:

    GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, Dhakal S, Wu M, Chen S, Feinberg T, Huang J, Saci A, Widlund HR, Fisher DE, Xiao Y, Rimm DL, Protopopov A, Wong KK, Chin L. Nature. 2009 Jun 25. 459(7250):1085-90. 10.1038/nature08109 PubMed 19553991
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