Ubc-c005
(Plasmid
#216876)
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PurposeBacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNIC-YFP
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUbiquitin
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SpeciesH. sapiens (human)
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Entrez GeneUBC (a.k.a. HMG20)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pNIC-YFP that contains the sequence of ubiquitin after the TEV-protease cleavage site
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ubc-c005 was a gift from Lari Lehtiö (Addgene plasmid # 216876 ; http://n2t.net/addgene:216876 ; RRID:Addgene_216876)