PARP1-c009
(Plasmid #216872)

• Purpose: Bacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusion
• Depositing Lab: Lari Lehtiö
• Publication: Lehtio lab accepted manuscripts (unpublished)

Backbone

• Vector backbone: pNIC-CFP
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Parp1-c009 needs 2 mM 3-amino benzamide inhibitor at all steps in the growth media and on plates. Bacteria will grow more slowly in the presence of inhibitor and will need more time for growth. Good mixing and aeration is important for growth in liquid cultures.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PARP1 catalytic domain (no Regulatory Domain)
• Species: H. sapiens (human)
• Entrez Gene: PARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)

Cloning Information

• Cloning method: Ligation Independent Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CFP-TEV site-PARP1 cat (no RD), PARP inhibitor 3-Amino Benzamide (2 mM) is required during growth.

Plasmid mutates easily and depositor recommends routine sequencing of at least the catalytic domain for each miniprep that you prepare.