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pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)
(Plasmid #216871)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216871 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9, gRNA Long.L, gRNA Long.R
  • gRNA/shRNA sequence
    unknown
  • Species
    M. musculus (mouse)
  • Mutation
    mdx mutation in mouse dystrophin
  • Entrez Gene
    Dmd (a.k.a. DXSmh7, DXSmh9, Dp427, Dp71, dys, mdx, pke)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right) was a gift from Ori Bar-Nur (Addgene plasmid # 216871 ; http://n2t.net/addgene:216871 ; RRID:Addgene_216871)

  • For your References section:

    Generation of allogenic and xenogeneic functional muscle stem cells for intramuscular transplantation. Lenardic A, Domenig SA, Zvick J, Bundschuh N, Tarnowska-Sengul M, Furrer R, Noe FJ, Trautmann CLL, Ghosh A, Bacchin G, Gjonlleshaj P, Qabrati X, Masschelein E, De Bock K, Handschin C, Bar-Nur O. J Clin Invest. 2024 May 7:e166998. doi: 10.1172/JCI166998. 10.1172/JCI166998 PubMed 38713532
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