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pBABE-HA-GOLPH3
(Plasmid #21687)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21687 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-PURO
  • Backbone size w/o insert (bp) 897
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Golgi Phosphoprotein 3
  • Alt name
    GOLPH3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    897
  • GenBank ID
    NM_022130
  • Entrez Gene
    GOLPH3 (a.k.a. GOPP1, GPP34, MIDAS, Vps74)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-HA-GOLPH3 was a gift from Lynda Chin (Addgene plasmid # 21687 ; http://n2t.net/addgene:21687 ; RRID:Addgene_21687)

  • For your References section:

    GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, Dhakal S, Wu M, Chen S, Feinberg T, Huang J, Saci A, Widlund HR, Fisher DE, Xiao Y, Rimm DL, Protopopov A, Wong KK, Chin L. Nature. 2009 Jun 25. 459(7250):1085-90. 10.1038/nature08109 PubMed 19553991
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