pEGFP-hTipin (pc4732)
(Plasmid
#216862)
-
PurposeMammalian expression vector encoding GFP tagged human Tipin
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 216862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepENeGFPRPA34 (pc624)
- Total vector size (bp) 5208
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTipin
-
SpeciesH. sapiens (human)
-
Insert Size (bp)910
-
Entrez GeneTIPIN
- Promoter CMV
-
Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEGFP-hTipin (pc4732) was a gift from Cristina Cardoso (Addgene plasmid # 216862 ; http://n2t.net/addgene:216862 ; RRID:Addgene_216862) -
For your References section:
Timeless-Tipin interactions with MCM and RPA mediate DNA replication stress response. Prorok P, Wolf E, Cardoso MC. Front Cell Dev Biol. 2024 Feb 29;12:1346534. doi: 10.3389/fcell.2024.1346534. eCollection 2024. 10.3389/fcell.2024.1346534 PubMed 38487270