RIX-F2Oct4F3-GFP
(Plasmid
#216848)
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PurposeThis plasmid is for the production of lentiviral vectors that can be used to track pluripotent stem cells, such as ES cells, iPS and tissue-resident pluripotent cells. GFP expression is lost upon diff
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216848 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRIX lentiviral vector
- Backbone size w/o insert (bp) 11000
- Total vector size (bp) 11778
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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Insert Size (bp)700
- Promoter Oct4
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SanDI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer gcccgaaggaatagaagaag
- 3′ sequencing primer cagctcgaccaggatgggcac (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RIX-F2Oct4F3-GFP was a gift from Patrick Salmon (Addgene plasmid # 216848 ; http://n2t.net/addgene:216848 ; RRID:Addgene_216848)
