pHAGE-FKBP-GFP-SINTBAD
(Plasmid
#216840)
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PurposeStably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE
- Total vector size (bp) 9700
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSINTBAD
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SpeciesH. sapiens (human)
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Entrez GeneTBKBP1 (a.k.a. ProSAPiP2, SINTBAD)
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Tag
/ Fusion Protein
- FKBP-GFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Fw (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the SINTBAD sequence is recombination prone. Carefully monitor the insert sequence at different steps of your protocol.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE-FKBP-GFP-SINTBAD was a gift from Sascha Martens (Addgene plasmid # 216840 ; http://n2t.net/addgene:216840 ; RRID:Addgene_216840) -
For your References section:
Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD. Adriaenssens E, Nguyen TN, Sawa-Makarska J, Khuu G, Schuschnig M, Shoebridge S, Watts EM, Csalyi KD, Padman BS, Lazarou M, Martens S. bioRxiv September 25, 202 10.1101/2023.09.25.559255