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p35S-FHA-AtAGO4_gDNA
(Plasmid #216838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216838 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pB7WG2
  • Total vector size (bp) 14133
  • Vector type
    Plant Expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARGONAUTE4
  • Alt name
    AGO4
  • Alt name
    AT2G27040
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    4706
  • Entrez Gene
    AGO4 (a.k.a. AT2G27040, ARGONAUTE 4, OCP11, OVEREXPRESSOR OF CATIONIC PEROXIDASE 11, T20P8.9, T20P8_9)
  • Promoter CaMV 35S
  • Tag / Fusion Protein
    • Flag-HA (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer AAAAGCAGGCTTCATGGAT
  • 3′ sequencing primer CTCACACATTATTCTGGAGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.03.587901 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p35S-FHA-AtAGO4_gDNA was a gift from Arturo Marí-Ordóñez (Addgene plasmid # 216838 ; http://n2t.net/addgene:216838 ; RRID:Addgene_216838)

  • For your References section:

    Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza. Dombey R, Buendía-Ávila D, Barragán-Borrero V, Diezma-Navas L, Ponce-Mañe A, Vargas-Guerrero JM, Elias R, Marí-Ordóñez A. bioRxiv 2024.04.03.587901; doi: https://doi.org/10.1101/2024.04.03.587901 10.1101/2024.04.03.587901
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