pGEX4T1-GST-LC3B
(Plasmid
#216836)
-
PurposeFor expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216836 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGEX-4T1
- Total vector size (bp) 5300
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameLC3B (Gly exposed)
-
SpeciesH. sapiens (human)
-
Entrez GeneMAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
- Promoter TAC promoter
-
Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer LacOp-for
- 3′ sequencing primer GEX3 Rv (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGEX4T1-GST-LC3B was a gift from Sascha Martens (Addgene plasmid # 216836 ; http://n2t.net/addgene:216836 ; RRID:Addgene_216836) -
For your References section:
Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD. Adriaenssens E, Nguyen TN, Sawa-Makarska J, Khuu G, Schuschnig M, Shoebridge S, Watts EM, Csalyi KD, Padman BS, Lazarou M, Martens S. bioRxiv September 25, 202 10.1101/2023.09.25.559255
Map uploaded by the depositor.
