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pAV-puro-U6+27-F30-Tornado-Squash-TfR-IRE
(Plasmid #216829)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216829 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAV-puro-U6+27
  • Backbone size w/o insert (bp) 5587
  • Total vector size (bp) 5759
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tornado-Squash-TfR-IRE
  • Species
    Synthetic
  • Insert Size (bp)
    172
  • Promoter U6+27

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer GGAAGAGGGCCTATTTCCCAT
  • 3′ sequencing primer CTAGTCCTCGGCTCGAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV-puro-U6+27-F30-Tornado-Squash-TfR-IRE was a gift from Samie Jaffrey (Addgene plasmid # 216829 ; http://n2t.net/addgene:216829 ; RRID:Addgene_216829)

  • For your References section:

    Engineering acyclovir-induced RNA nanodevices for reversible and tunable control of aptamer function. Hagen T, Litke JL, Nasir N, Hou Q, Jaffrey SR. Cell Chem Biol. 2024 Aug 23:S2451-9456(24)00319-2. doi: 10.1016/j.chembiol.2024.07.017. 10.1016/j.chembiol.2024.07.017 PubMed 39191249
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