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Addgene

pCMV-GST-IRP1
(Plasmid #216827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ACO1
  • Alt name
    IRP1
  • Alt name
    IREB1
  • Alt name
    ACONS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2684
  • GenBank ID
    NM_002197.2
  • Entrez Gene
    ACO1 (a.k.a. ACONS, HEL60, IREB1, IREBP, IREBP1, IRP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GGCAAGCCACGTTTGGTG
  • 3′ sequencing primer TGTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-GST-IRP1 was a gift from Samie Jaffrey (Addgene plasmid # 216827 ; http://n2t.net/addgene:216827 ; RRID:Addgene_216827)
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