pDM098
(Plasmid #216818)

• Purpose: Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.
• Depositing Lab: Ken Sawin
• Publication: Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system (unpublished)

Backbone

• Vector backbone: pUC19
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mStayGold
• Species: Synthetic
• Mutation: Aspergillus nidulans codon-adjusted
• Tag / Fusion Protein:
  • FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • pDM098.gbk
• A portion of this plasmid was derived from a plasmid made by: Synthethic gene fragment from IDT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry