pDM068
(Plasmid
#216811)
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Purpose(Empty Backbone) Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216811 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFC330 (Addgene #87832)
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Vector typeBacterial Expression, CRISPR ; Fungal Expression
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Selectable markersNAT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySynthethic gene fragment from IDT
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Replaced Aspergillus fumigatus pyrG CDS pFC330 backbone with NAT CDS
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDM068 was a gift from Ken Sawin (Addgene plasmid # 216811 ; http://n2t.net/addgene:216811 ; RRID:Addgene_216811)
Map uploaded by the depositor.
