pDM030
(Plasmid #216810)

• Purpose: (Empty Backbone) Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
• Depositing Lab: Ken Sawin
• Publication: Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system (unpublished)

Backbone

• Vector backbone: pFC333 (Addgene #87844)
• Vector type: Bacterial Expression, CRISPR ; Fungal Expression
• Selectable markers: ble

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • pDM030.gbk
• A portion of this plasmid was derived from a plasmid made by: Synthethic gene fragment from IDT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry