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rSirt3 shRNA (pLKO.1 vector)
(Plasmid #216807)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216807 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1 - TRC cloning vector
  • Backbone manufacturer
    David Root
  • Backbone size w/o insert (bp) 7032
  • Total vector size (bp) 7080
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sirt3
  • Alt name
    Sirtuin 3
  • Alt name
    NAD-dependent protein deacetylase sirtuin-3
  • gRNA/shRNA sequence
    ACGTGCACGGTCTGTCGAA
  • Species
    R. norvegicus (rat)
  • GenBank ID
  • Entrez Gene
    Sirt3

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rSirt3 shRNA (pLKO.1 vector) was a gift from Ghazaleh Ashrafi (Addgene plasmid # 216807 ; http://n2t.net/addgene:216807 ; RRID:Addgene_216807)

  • For your References section:

    Sirtuin3 ensures the metabolic plasticity of neurotransmission during glucose deprivation. Tiwari A, Hashemiaghdam A, Laramie MA, Maschi D, Haddad T, Stunault MI, Bergom C, Javaheri A, Klyachko V, Ashrafi G. J Cell Biol. 2024 Jan 1;223(1):e202305048. doi: 10.1083/jcb.202305048. Epub 2023 Nov 21. 10.1083/jcb.202305048 PubMed 37988067
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