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Addgene

pGL6000
(Plasmid #216797)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL6000
  • Backbone size (bp) 8064
  • Vector type
    Cre/Lox ; Leishmania DiCre Inducible Expression
  • Promoter rRNA promoter
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • 6xHA (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL6000 was a gift from Jeremy Mottram (Addgene plasmid # 216797 ; http://n2t.net/addgene:216797 ; RRID:Addgene_216797)

  • For your References section:

    A DiCre recombinase-based system for inducible expression in Leishmania major. Santos RERS, Silva GLA, Santos EV, Duncan SM, Mottram JC, Damasceno JD, Tosi LRO. Mol Biochem Parasitol. 2017 Sep;216:45-48. doi: 10.1016/j.molbiopara.2017.06.006. Epub 2017 Jun 16. 10.1016/j.molbiopara.2017.06.006 PubMed 28629935
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