pCW57-Cx32D178Y-IRES-GCaMP6s
(Plasmid
#216796)
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PurposeInducible bicistronic lentiviral plasmid for the simultaneous expression of the D178Y mutant form of Connexin 32 and cytosolic GCaMP6s
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCW57.1
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Vector typeLentiviral
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Selectable markersPuromycin ; None
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCx32D178Y-IRES-GCaMP6s
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SpeciesH. sapiens (human)
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MutationD178Y mutant form of Connexin 32
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Entrez GeneGJB1 (a.k.a. CMTX, CMTX1, CX32)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCW57-Cx32D178Y-IRES-GCaMP6s was a gift from Fabio Mammano (Addgene plasmid # 216796 ; http://n2t.net/addgene:216796 ; RRID:Addgene_216796)