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pCW57-Cx32D178Y-IRES-GCaMP6s
(Plasmid #216796)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216796 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin ; None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cx32D178Y-IRES-GCaMP6s
  • Species
    H. sapiens (human)
  • Mutation
    D178Y mutant form of Connexin 32
  • Entrez Gene
    GJB1 (a.k.a. CMTX, CMTX1, CX32)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57-Cx32D178Y-IRES-GCaMP6s was a gift from Fabio Mammano (Addgene plasmid # 216796 ; http://n2t.net/addgene:216796 ; RRID:Addgene_216796)

  • For your References section:

    A fully human IgG1 antibody targeting connexin 32 extracellular domain blocks CMTX1 hemichannel dysfunction in an in vitro model. Tettey-Matey A, Donati V, Cimmino C, Di Pietro C, Buratto D, Panarelli M, Reale A, Calistri A, Fornaini MV, Zhou R, Yang G, Zonta F, Marazziti D, Mammano F. Cell Commun Signal. 2024 Dec 5;22(589). doi: 10.1186/s12964-024-01969-0. 10.1186/s12964-024-01969-0
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