pCW57-Cx32-IRES-GCaMP6s
(Plasmid
#216795)
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PurposeInducible bicistronic lentiviral plasmid for the simultaneous expression of Connexin 32 and cytosolic GCaMP6s
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216795 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCW57.1
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Vector typeLentiviral
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Selectable markersPuromycin ; None
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCW57-Cx32-IRES-GCaMP6s was a gift from Fabio Mammano (Addgene plasmid # 216795 ; http://n2t.net/addgene:216795 ; RRID:Addgene_216795) -
For your References section:
A fully human IgG1 antibody targeting connexin 32 extracellular domain blocks CMTX1 hemichannel dysfunction in an in vitro model. Tettey-Matey A, Donati V, Cimmino C, Di Pietro C, Buratto D, Panarelli M, Reale A, Calistri A, Fornaini MV, Zhou R, Yang G, Zonta F, Marazziti D, Mammano F. Cell Commun Signal. 2024 Dec 5;22(589). doi: 10.1186/s12964-024-01969-0. 10.1186/s12964-024-01969-0