pBABE FLAG-IKK2-EE puro
(Plasmid #216790)

• Purpose: Mammalian expression of constitutively active human IKK-2
• Depositing Lab: Kevin Janes
• Publication: Janes lab plasmids (unpublished)

Backbone

• Vector backbone: pBABE puro
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IKK-2
• Species: H. sapiens (human)
• Insert Size (bp): 2310
• Mutation: S177E and S181E, constitutively active
• Entrez Gene: IKBKB (a.k.a. IKK-2, IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB)
• Promoter: LTR
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was created by PCR cloning IKK2-EE (Addgene #11105) using BclI (5') and MfeI (3') and inserting into pBABE puro that had been digested by BamHI (5') and EcoRI (3')