pEN_TT CVB3 A67G
(Plasmid #216788)

• Purpose: Gateway entry vector for the Kandolf strain of CVB3 with a VP4 A67G mutation
• Depositing Lab: Kevin Janes
• Publication: Janes lab plasmids (unpublished)

Backbone

• Vector backbone: pEN_TTmcs
• Vector type: Entry vector for gateway cloning

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CVB3
• Species: Coxsackievirus B3
• Insert Size (bp): 6558
• Mutation: A67G in VP4

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To maintain the CVB3 internal EcoRI site, this plasmid was created by in a two step procedure: i) PCR cloning of CVB3 from its internal EcoRI site (nucleotides 2764-2769) to the end (nucleotide 7399), digesting with EcoRI-MfeI, and ligating into pEN_TTmcs (Addgene #25755) digested with EcoRI; ii) PCR cloning of CVB3 from its start (nucleotide 1) to the internal EcoRI site, digesting with EcoRI, and ligating into the intermediate plasmid (i) digested with EcoRI.