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Addgene

pEN_TT CVB3 A67G
(Plasmid #216788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEN_TTmcs
  • Vector type
    Entry vector for gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CVB3
  • Species
    Coxsackievirus B3
  • Insert Size (bp)
    6558
  • Mutation
    A67G in VP4

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To maintain the CVB3 internal EcoRI site, this plasmid was created by in a two step procedure: i) PCR cloning of CVB3 from its internal EcoRI site (nucleotides 2764-2769) to the end (nucleotide 7399), digesting with EcoRI-MfeI, and ligating into pEN_TTmcs (Addgene #25755) digested with EcoRI; ii) PCR cloning of CVB3 from its start (nucleotide 1) to the internal EcoRI site, digesting with EcoRI, and ligating into the intermediate plasmid (i) digested with EcoRI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN_TT CVB3 A67G was a gift from Kevin Janes (Addgene plasmid # 216788 ; http://n2t.net/addgene:216788 ; RRID:Addgene_216788)
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