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Addgene

pJF6
(Plasmid #21678)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21678 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJH271
  • Vector type
    centromeric plasmid
  • Selectable markers
    LEU2, URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LacZ
  • Alt name
    2 direct repeat .copies of lacZ
  • Alt name
    see author's map
  • Species
    E. coli
  • Mutation
    HO digestion site in one of the LacZ genes
  • Entrez Gene
    lacZ (a.k.a. ECIAI39_0334)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and
LacZ::CS cut site was inserted into the HindIII site at the
upstream end of a promotorless LacZ gene on plasmid JH271.

The two LacZ sequences are in direct repeat orientation relative to each other.

The lambda sequences are of unknown origin

Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJF6 was a gift from James Haber (Addgene plasmid # 21678 ; http://n2t.net/addgene:21678 ; RRID:Addgene_21678)

  • For your References section:

    Two alternative pathways of double-strand break repair that are kinetically separable and independently modulated. Fishman-Lobell J, Rudin N, Haber JE. Mol Cell Biol. 1992 Mar . 12(3):1292-303. PubMed 1545810
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