mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
(Plasmid #216763)

• Purpose: PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase
• Depositing Lab: Alex Dunn
• Publication: Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. doi: 10.1021/acssensors.3c02664. Epub 2024 Jul 7.

Backbone

• Vector backbone: PiggyBac
• Backbone size w/o insert (bp): 7600
• Total vector size (bp): 13200
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
• Species: G. gallus (chicken), Synthetic
• Insert Size (bp): 5600
• Tags / Fusion Proteins:
  • mCherry (N terminal on backbone)
  • EGFP (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • mCherry-LgBiT-tPT2A-EGFP-Vinculin-PILATeS-CT_NoTransposasePB.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This PILATeS module contains BiT tag with 700 pM affinity for LgBiT. PILATeS is appended C-terminally to vinculin as an unloaded negative control. Requires co-transfection with transposase