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mCh-LgBiT-2A-EGFP-Vin-PILATeS
(Plasmid #216761)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216761 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Backbone size w/o insert (bp) 7600
  • Total vector size (bp) 13200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
  • Species
    G. gallus (chicken), Synthetic
  • Insert Size (bp)
    5600
  • Tags / Fusion Proteins
    • mCherry (N terminal on backbone)
    • EGFP (N terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This PILATeS module contains BiT tag with 700 pM affinity for LgBiT. Requires co-transfection with transposase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCh-LgBiT-2A-EGFP-Vin-PILATeS was a gift from Alex Dunn (Addgene plasmid # 216761 ; http://n2t.net/addgene:216761 ; RRID:Addgene_216761)

  • For your References section:

    Split Luciferase Molecular Tension Sensors for Bioluminescent Readout of Mechanical Forces in Biological Systems. Zhong BL, Elliot JM, Wang P, Li H, Hall RN, Wang B, Prakash M, Dunn AR. ACS Sens. 2024 Jul 26;9(7):3489-3495. doi: 10.1021/acssensors.3c02664. Epub 2024 Jul 7. 10.1021/acssensors.3c02664 PubMed 38973210
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