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Addgene

pCAX-SCLM
(Plasmid #216757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAX
  • Backbone size w/o insert (bp) 4287
  • Total vector size (bp) 5682
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SuperClomeleon
  • Alt name
    Cerulean/S30R/dC11-LE-Topaz/dN5/S30R/Q69T/V163A
  • Species
    A. victoria (crystal jelly)
  • Insert Size (bp)
    1395
  • Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiety and 5 AA deletion, S30R, Q69T, and V163A in the Topaz YFP moiety
  • Promoter CAG (cytomegalovirus-chicken beta-actin-rabbit beta-globin)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer tgctggttattgtgctgtctcatc
  • 3′ sequencing primer ATATGTTGCCAAACTCTAAACC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAX-SCLM was a gift from George Augustine (Addgene plasmid # 216757 ; http://n2t.net/addgene:216757 ; RRID:Addgene_216757)
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